EC Number   |
|---|
    3.8.1.2 | - |
| 3.8.1.2 | alpha/beta structure |
| 3.8.1.2 | apoenzyme and enzyme in intermediate state with substrate L-2-monochloropropionate and monochloroacetate |
| 3.8.1.2 | crystal structures of enzyme complexed with monochloroacetate, L-2-chlorobutyrate, L-2-chloro-3-methylbutyrate, or L-2-chloro-4-methylvalerate are determined using the complex crystals prepared with the S175A mutant |
| 3.8.1.2 | mutant S175A |
| 3.8.1.2 | mutant S175A in complex with chloroacetate, 2-L-chlorobutyrate, 2-L-chloro-3-methylbutyrate, 2-L-chloro-4-methylvalerate, and L-2-chloropropionamide, all substrates bound have D-configuration, except for chloroacetate |
| 3.8.1.2 | oil-microbatch method, in 200 mM NaCl and 20 mM Tris-HCl pH 8.0, 25.5% (w/v) polyethylene glycol 4000, 0.17 M ammonium acetate, 15% (w/v) glycerol and 85 mM sodium acetate buffer, pH 4.6, at 20°C |
| 3.8.1.2 | refined to 1.9 A resolution. Crystal is an orderdisorder twin by reticular merohedry with a twin index of 10 |
| 3.8.1.2 | three-dimensional structure of enzyme |
