  3.5.99.8 | selenomethionine-labeled apo 5NAA-A, substrate-bound 5NAA-A, product- and Mn2+-bound 5NAA-A, and the Meisenheimer complex intermediate with Zn2+ bound to the 5NAA-AR289A mutant, sitting drop method, room temperature, X-ray diffraction structure determination and analysis. 9.4 mg/ml SeMet apo 5NAA-A protein in 50 mM bicine, pH 7.0, and 150 mM NaCl crystallizes from 1.07 M NaH2PO4, and 0.83 M K2HPO4. 7 mg/ml wild-type 5NAA-A protein in 50 mM bicine, pH 7.0, 150 mM NaCl, and 5NAA (0.22 mM) crystallizes from 1.27 M NaH2PO4, and 0.63 M K2HPO4. 5NAA-A-Mn2+ complex from 5.8 mg/ml protein in 50 mM bicine, pH 7.0, 150 mM NaCl, 16 mM MnCl2, and 0.16 mM 5NSA crystallizes from 0.1 M sodium cacodylate pH 6.5, and 0.75 M trisodium citrate. 5.8 mg/ml mutant 5NAA-AR289A protein in 50 mM bicine, pH 7.0, 150 mM NaCl, 12 mM Zn(OAc)2, and 0.22 mM 5NAA crystallizes from 0.1 M sodium cacodylate, pH 6.5, and 0.65 M trisodium citrate |
