EC Number   |
|---|
   3.2.1.96 | at 1.9 A resolution |
| 3.2.1.96 | CBM32 (a family 32 carbohydrate-binding module of endo-beta-1,4-N-acetylglucosamidase), hanging drop vapor diffusion method, using 30% (w/v) PEG 4000, 0.2 M ammonium acetate and 0.1 M sodium citrate pH 6.5 |
| 3.2.1.96 | Endo-A in the native form or in complex with Man3GlcNAc-thiazoline or GlcNAc-Asn, between 2-3.5 A resolution. Crystals of Endo-A belong to P1 space group with four molecules of the protein in the asymmetric unit. The carbohydrate moiety sits above the TIM-barrel in a cleft region surrounded by aromatic residues. N171 is hydrogen bonded to the thiazoline nitrogen, mimicking the ability of the asparagine to orient the acetamido group for a nucleophilic attack on the anomeric carbon |
| 3.2.1.96 | hanging drop vapor diffusion method, complexed with Gal-beta(1-4)-GlcNAc-beta(1-2)-Man-alpha(1-3)[Gal-beta(1-4)-GlcNAc-beta(1-2)-Man-alpha(1-6)]Man-beta(1-4)-GlcNAc |
| 3.2.1.96 | purifed recombinant wild-type enzyme and mutant E173Q in unlabeled or selenomethionine-labeled forms, 1:1 mixture of 42 mg/mL protein with a reservoir solution consisting of 0.35 M KSCN, 0.1 M 1,3-bis(tris(hydroxymethyl)methylamino)propane, pH 6.5, and 15% w/v PEG 3350, 30 days at 4 °C for the unlabeled, and 14 days at 18 °C for the SeM-labeled enzymes, X-ray diffraction and analysis at 1.8 A resolution, multiple-wavelength anomalous scattering methods, structure modelling, overview |
| 3.2.1.96 | wild-type enzyme and mutant enzymes D130N, D130E, D130A, E132Q, E132A, and D130N/E132Q |
