EC Number   |
|---|
   3.1.21.2 | hanging drop vapor diffusion method, using 100 mM Tris-HCl, pH 9.0, 4 mM dithiothreitol, 1.5% saturated MgSO4, 16% (w/v) ethylene glycol, 20% (w/v) polyethylene glycol monomethyl ether 2000 |
| 3.1.21.2 | mutant E118A of EndoII is crystallized in space group P21 with four monomers in the asymmetric unit, with a bound phosphate ion in one of the four active sites of EndoII likely mimicing the scissile phosphate in a true substrate complex. Crystallization of the selenomethionine-substituted E118A mutant, X-ray diffraction structure determination and analysis at 1.9-2.3 A resolution, molecular replacement |
| 3.1.21.2 | sitting-drop vapor-diffusion method at 18°C, the structure of the recombinant endonuclease IV is determined at 1.18 A resolution |
| 3.1.21.2 | using the sitting-drop vapor-diffusion method |
| 3.1.21.2 | wild-type enzyme and mutant H69A, hanging-drop vapor diffusion method, mxing of 0.0015 ml of protein solution containing 10 mg/ml protein with 0.002 ml of reservoir solution containing 35% PEG 400 and 100 mM HEPES, pH 5.5, 20°C, 7 days, X-ray diffraction structure determination and analysis at 1.55 A resolution |
