2.6.1.37 | crystals of Se-Met containing 2-aminoethylphosphonate-pyruvate transaminase, hanging drop vapor diffusion at 4°C, enzyme solution containing 0.45 mM protein, 8 mM phosphate buffer, pH 7.5, 0.8 mM dithiothreitol and 20 mM phosphonacetaldehyde is equilibrated against a reservoir solution containing 200 mM ammonium acetate, 100 mM sodium citrate, pH 5.0, and 10-13% monomethyl polyethylene glycol 5000, yellow crystals appear within 2 weeks |
636710 |