1.3.8.12 | purified recombinant FAD-bound enzyme, sitting drop vapor diffusion method, mixing of 20 mg/ml protein in 20 mM Tris-HCl, pH 7.9, 200 mM NaCl, 1.5 mM FAD, and 3 mM mesaconyl-CoA in an 1:1 ratio with crystallization solution containing 30% PEG 5000 monomethyl ether mesylate, 100 mM Tris, pH 8.0, and 200 mM LiSO4, 16°C, X-ray diffraction structure determination and analysis at 1.37 A resolution, modeling |