EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
6.1.1.5 | 0.03 |
- |
tRNAIle |
pH 7.5, 30°C, deacylation, recombinant mutant D334A |
745362 |
6.1.1.5 | 0.038 |
- |
AMP |
pH 7.5, 30°C, deacylation, recombinant mutant D334A |
745362 |
6.1.1.5 | 0.13 |
- |
tRNAIle |
pH 7.5, 30°C, deacylation, recombinant wild-type enzyme |
745362 |
6.1.1.5 | 0.18 |
- |
L-isoleucine |
pH 7.5, recombinant isozyme IleRS-R1 |
652415 |
6.1.1.5 | 0.19 |
- |
tRNAIle |
pH 7.5, 30°C, deacylation, recombinant mutant D334A |
745362 |
6.1.1.5 | 0.2 |
- |
AMP |
pH 7.5, 30°C, deacylation, recombinant mutant D334A |
745362 |
6.1.1.5 | 0.2 |
- |
AMP |
pH 7.5, 30°C, deacylation, recombinant wild-type enzyme |
745362 |
6.1.1.5 | 0.29 |
- |
ATP |
pH 7.5, 37°C, mutant T243R/D342A, in presence of tRNA |
715519 |
6.1.1.5 | 0.35 |
- |
L-isoleucine |
tRNAIle aminoacylation reaction, pH 7.9, 22°C |
651903 |
6.1.1.5 | 0.48 |
- |
ATP |
pH 7.5, 37°C, mutant D342A, in presence of tRNA |
715519 |