EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    3.2.1.22 | -999 |
- |
phenyl alpha-D-galactoside |
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C |
678262 |
| 3.2.1.22 | 0.00218 |
- |
4-nitrophenyl alpha-D-galactoside |
mutant enzyme D415N, in 40 mM sodium phosphate buffer, pH 6.0, at 35°C |
705156 |
| 3.2.1.22 | 0.01 |
- |
3,4-dimethylphenyl alpha-D-galactoside |
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C |
678262 |
| 3.2.1.22 | 0.01 |
- |
4-O-methylphenyl alpha-D-galactoside |
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C |
678262 |
| 3.2.1.22 | 0.016 |
- |
4-bromophenyl alpha-D-galactoside |
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C |
678262 |
| 3.2.1.22 | 0.021 |
- |
beta-naphthyl alpha-D-galactoside |
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C |
678262 |
| 3.2.1.22 | 0.027 |
- |
phenyl alpha-D-galactoside |
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C |
678262 |
| 3.2.1.22 | 0.03 |
- |
4-nitrophenyl alpha-D-galactoside |
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C |
678262 |
| 3.2.1.22 | 0.03 |
- |
melibiose |
pH 5.0, 55°C, intracellular enzyme |
698429 |
| 3.2.1.22 | 0.03 |
0.55 |
4-O-methylphenyl alpha-D-galactoside |
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C |
678262 |
