EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
2.6.1.16 | 0.00092 |
- |
L-gamma-glutamyl-p-nitroanilide |
wild-type with N-terminal His-tag, pH 7.5, 25°C |
671896 |
2.6.1.16 | 0.004 |
- |
D-fructose 6-phosphate |
hexose phosphate-isomerizing activity, wild-type with C-terminal His-tag, pH 7.5, 25°C |
671896 |
2.6.1.16 | 0.0075 |
- |
L-gamma-glutamyl-p-nitroanilide |
residues 1-345, His-tagged glutamine amide-hydrolysing domain, pH 7.5, 25°C |
671896 |
2.6.1.16 | 0.0092 |
- |
D-fructose 6-phosphate |
hexose phosphate-isomerizing activity, residues 346-712, His-tagged hexose phosphate-isomerizing domain, pH 7.5, 25°C |
671896 |
2.6.1.16 | 0.012 |
- |
L-gamma-glutamyl-p-nitroanilide |
wild-type, pH 7.5, 25°C |
671896 |
2.6.1.16 | 0.0123 |
- |
L-gamma-glutamyl-p-nitroanilide |
reaction mixture contains 1 mM L-gamma-glutamyl-p-nitroanilide, 1 mM EDTA, 1 mM DTT and the appropriately diluted enzyme preparation in 20 mM HEPES buffer (pH 7.5) at 25°C |
671896 |
2.6.1.16 | 0.013 |
- |
L-gamma-glutamyl-p-nitroanilide |
wild-type with C-terminal His-tag, pH 7.5, 25°C |
671896 |
2.6.1.16 | 0.021 |
- |
D-fructose 6-phosphate |
hexose phosphate-isomerizing activity, wild-type with N-terminal His-tag, pH 7.5, 25°C |
671896 |
2.6.1.16 | 0.021 |
- |
D-fructose 6-phosphate |
hexose phosphate-isomerizing activity, wild-type, pH 7.5, 25°C |
671896 |
2.6.1.16 | 0.0213 |
- |
D-fructose 6-phosphate |
reaction mixture contains 2 mM D-fructose 6-phosphate, 0.5 mM NADP+, 1 mM EDTA, and appropriately diluted GlcN-6-P synthase preparation in 50 mM Tris/HCl buffer (pH 7.5) at 25°C |
671896 |