EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
2.1.1.28 | 0.00017 |
- |
phenylethanolamine |
mutant E185Q, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C. |
672011 |
2.1.1.28 | 0.001 |
- |
3-trifluoromethylphenylethanolamine |
mutant D267A |
675288 |
2.1.1.28 | 0.0017 |
- |
S-adenosyl-L-methionine |
mutant D267N |
675288 |
2.1.1.28 | 0.0028 |
- |
phenylethanolamine |
mutant E185A, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C. |
672011 |
2.1.1.28 | 0.0043 |
- |
anti-9-amino-6-(trifluoromethyl)benzonorbornene |
mutant D267A |
675288 |
2.1.1.28 | 0.0048 |
- |
cis-(1R,2S)-2-amino-1-tetralol |
wild type enzyme, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C. |
672011 |
2.1.1.28 | 0.0052 |
- |
S-adenosyl-L-methionine |
mutant D267A |
675288 |
2.1.1.28 | 0.0062 |
- |
anti-9-amino-6-(trifluoromethyl)benzonorbornene |
wild type enzyme |
675288 |
2.1.1.28 | 0.008 |
- |
3-trifluoromethylphenylethanolamine |
pH 8.0, 30°C |
733211 |
2.1.1.28 | 0.0083 |
- |
3-trifluoromethylphenylethanolamine |
wild type enzyme |
675288 |