EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
4.1.3.1 | -999 |
- |
biphasic kinetics of thermal inactivation |
33129 |
4.1.3.1 | -999 |
- |
KCl, MgCl2, CaCl2 stabilize |
33134, 33135 |
4.1.3.1 | -999 |
- |
more heat labile at low concentrations |
33121 |
4.1.3.1 | -999 |
- |
oxalate protects |
33125 |
4.1.3.1 | -999 |
- |
the amino acid residue at position 504 is involved in thermostability of the ICL enzymes |
727196 |
4.1.3.1 | 25 |
- |
no loss of activity after 30 min |
33148 |
4.1.3.1 | 30 |
- |
16% loss of activity after 30 min |
33148 |
4.1.3.1 | 30 |
- |
complete inactivation after 2 min |
651215 |
4.1.3.1 | 30 |
- |
enzyme is rapidly inactivated above, NaCl, 10% glycerol and 1 mM dithiothreitol protects |
638504 |
4.1.3.1 | 30 |
- |
mutant Q207H, half time of thermal inactivation 58.4 s |
666268 |