EC Number   |
|---|
   5.4.3.11 | a 1639 bps long synthetic gene, recombinant overexpression of N-terminally His10-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS, real-time PCR enzyme expression analysis, method evaluation, optimization, and upscaling to high level protein expression. 37°C is the best temperature for maximum protein production. Subcloning in Escherichia coli strain XL-1 Blue |
| 5.4.3.11 | expression in Escherichia coli |
| 5.4.3.11 | gene hitA, cDNA library construction, genome mining of hitachimycin biosynthetic gene cluster, DNA and amino acid sequence determination and analysis. The identified gene cluster is responsible for the biosynthesis of hitachimycin. Recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 5.4.3.11 | gene pam, functional recombinant expression from plasmid pET28a in Escherichia coli strain BL21 |
| 5.4.3.11 | gene papam, recombinant expression of C-terminally His6-tagged PaPAM in Escherichia coli strain BL21(DE3) |
| 5.4.3.11 | recombinant expression of N-terminally His10-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS |
