EC Number |
---|
3.5.4.9 | - |
3.5.4.9 | 6His-tagged enzyme is expressed in Escherichia coli |
3.5.4.9 | amplified genomic library transformed into yest YA3-1, yeast-DNA transformed into Escherichia coli K38 pBKe, gene folD encoding PPDC expressed |
3.5.4.9 | bifunctional dehydrogenase/cyclohydrolase portion of the cytoplasmic trifunctional enzyme cloned and heterologous expressed in the yeast strain YA3-1 |
3.5.4.9 | cDNA encoding bifunctional enzyme cloned and expressed in Escherichia coli K38 |
3.5.4.9 | cDNA encoding trifunctional enzyme used to complete the purine auxotrophic yeast strain YA3-1 |
3.5.4.9 | deletion of the MIS1 gene has little effect |
3.5.4.9 | DNA fragment containing the bifunctional enzyme from Escherichia coli BE cloned and transformed into Escherichia coli BL21 for protein expression |
3.5.4.9 | expressed in Escherichia coli BL21(DE3) cells |
3.5.4.9 | expressed in Escherichia coli strain BL21 (DE3) |