EC Number |
---|
3.2.1.31 | - |
3.2.1.31 | beta-D-glucuronidase gene derived from Penicillium purpurogenum Li-3 is expressed in Pichia pastoris GS115 in two different environments of low-shear modeled microgravity (LSMMG) and normal gravity (NG) |
3.2.1.31 | DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree |
3.2.1.31 | expressed in 3T3 fibroblasts and EJ carcinoma cells |
3.2.1.31 | expressed in Escherichia coli |
3.2.1.31 | expressed in Escherichia coli DH5alpha cells |
3.2.1.31 | expressed in Escherichia coli strain JM109 |
3.2.1.31 | expressed in Escherichia coli strain Origami B(DE3) |
3.2.1.31 | expression in both Escherichia coli and Arabidopsis thaliana. The enzyme expressed in transformed Escherichia coli cells exhibits maximum hydrolytic activity at 65°C and pH 6.5 and retains more than 80% activity after incubation at 85°C for 30 min. Activity in transgenic Arabidopsis thaliana plants containing TmGUSI is also stable over the temperature range 65-80°C |
3.2.1.31 | expression in Escherichia coli |