EC Number |
---|
3.1.1.20 | - |
3.1.1.20 | 1.4 kb purified PCR product of tanLp1 gene cloned into pURI3 vector and transformed into Escherichia coli DH5alpha cells. Recombinant plasmids isolated and overexpressed in Escherichia coli JM109 (DE3) |
3.1.1.20 | cloned in Escherichia coli JM 109 and expressed in Aspergillus oryzae strain AO1, a tannase low-producing and nitrate reductase-deficient strain |
3.1.1.20 | DNA and amino acid sequence determination and analysis, expressionin Escherichia coli strain DH5alpha |
3.1.1.20 | DNA and amino acid sequence determination nand analysis, sequence comparisons and phylogenetic tree, recombinant expression in Pichia pastoris strain KM71 of the tannase, without the signal sequence, but with secretion signal sequence of Saccharomyces cerevisiae instead of the original signal sequence, the enzyme is secreted |
3.1.1.20 | expressed in Arxula adeninivorans strain G1212 |
3.1.1.20 | expressed in Escherichia coli BL21(DE3) and B834(DE3) cells |
3.1.1.20 | expressed in Escherichia coli BL21(DE3) cells |
3.1.1.20 | expressed in Escherichia coli BL21-AI cells |
3.1.1.20 | expressed in Escherichia coli DH5alpha |