EC Number |
---|
2.6.1.83 | - |
2.6.1.83 | cDNA sequence of LL-DAP-AT from Arabidopsis thaliana is optimized for expression in bacteria and cloned in Escherichia coli without its leader sequence but with a C-terminal hexahistidine affinity tag to aid protein purification |
2.6.1.83 | cloned in Escherichia coli without its leader sequence but with a C-terminal hexahistidine affinity tag to aid protein purification |
2.6.1.83 | DNA chemically synthesized, His-tagged protein expressed in Escherichia coli M15(pREP4) and Escherichia coli BL21(DE3) |
2.6.1.83 | expressed in Escherichia coli |
2.6.1.83 | expressed in Escherichia coli Origami 2(DE3), also used in complementation experiments |
2.6.1.83 | expression in Escheirchia coli |
2.6.1.83 | gene DAP, genotyping |
2.6.1.83 | gene dapL, functional recombinant expression of the enzyme in Escherichia coli double mutant strain DELTAdapD/E and complementation, and functional recombinant overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) |
2.6.1.83 | His-tagged protein (amino acid residue 21-456) expressed in Escherichia coli BL21(DE3) |