EC Number |
---|
2.4.2.19 | - |
2.4.2.19 | expressed in Escherichia coli as glutathione S-transferase fusion protein |
2.4.2.19 | expressed in Escherichia coli BL21(DE3) cells |
2.4.2.19 | expressed in Escherichia coli BL21(DE3)-RIPL Codon Plus |
2.4.2.19 | expressed in Escherichia coli TH265 (nadC mutant)(complementation approach) |
2.4.2.19 | expression in Escherichia coli |
2.4.2.19 | gene TM1645, expression of N-terminally His-tagged enzyme in Escherichia coli |
2.4.2.19 | His-tagged protein expressed in Escherichia coli C41 (DE3) |
2.4.2.19 | in pEHISTEV (pBS-hQPRT as template) for expression in Escherichia coli BL21(DE3) or mutagenesis, expression with N-terminal hexa-His tag and TEV protease cleavage site, two extra N-terminal residues (Gly, Ala) remain after removal of hexa-His tag |
2.4.2.19 | into plasmid pTYB12 and expressed in Escherichia coli BL21 |