EC Number   |
|---|
    2.4.2.14 | - |
| 2.4.2.14 | amplified from seedling cDNA library to encode 11-amino acid autoprocessing propeptide, N-terminal cysteine for an active enzyme and deleted chloroplast transit peptide, in pCR2.1TOPO for sequencing, in pET26b for expression in Escherichia coli BL21(DE3) |
| 2.4.2.14 | expressed in Escherichia coli BL21 cells |
| 2.4.2.14 | expression in Escherichia coli |
| 2.4.2.14 | expression of wild-type and K338Q/P422W mutant enzyme in amidophosphoribosyltransferase deficient CHO ade -A cells and in transgenic mice |
| 2.4.2.14 | expression of wild-type, Y74A, Y258A, Y258F, K326Q, Y329A, G331I, N351A and Y465A mutant enzyme in Escherichia coli |
| 2.4.2.14 | overexpression of wild-type and P410W mutant enzyme in Escherichia coli |
| 2.4.2.14 | used in gene replacment and complementation experiments |
| 2.4.2.14 | wildtype, mutant I198V and variants 1-04 and 2-02 of mutant I198V in pCA24N for expression as N-terminal hexa-His-tagged, C-terminal GFP-tagged proteins in Escherichia coli JMB9 DELTA TrpF (auxotrophic for tryptophan) and/or Escherichia coli KK8(pDM), mutant I198V in pCDF-1b for error-prone PCR based mutagenesis yielding variant 1-04 |
