EC Number   |
|---|
   2.4.1.170 | cloning of gene homologues GmIF7GT1-GmIF7GT6, DNA and amino acid sequence determination, expression analysis |
| 2.4.1.170 | DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 2.4.1.170 | expressed in Escherichia coli |
| 2.4.1.170 | expressed in Escherichia coli BL21(DE3) |
| 2.4.1.170 | gene GmIF7GT, DNA and amino acid sequence determination and analysis, expression analysis |
| 2.4.1.170 | gene GmIF7GT, recombinant functional expression in Escherichia coli strain BL21(DE3), coexpression with acyl-CoA carboxylase alpha and beta subunits, biotin ligase, and acetyl-CoA synthetase from Nocardia farcinia for subsequent malonylization of the reaction product of GmIF7GT-encoded isoflavone 7-O-glucosyltransferase. The isoflavonoids are glycosylated at position 7 by 7-O-glycosyltranferase and are further malonylated at position 6' of glucose by malonyl-CoA:isoflavone 7-O-glucoside-6'-O-malonyltransferase both from Glycine max |
| 2.4.1.170 | gene GmIF7GT1, quantitative real-time reverse transcription-PCR enzyme expression analysis |
| 2.4.1.170 | gene GmUGT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis |
| 2.4.1.170 | gene GmUGT3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis |
| 2.4.1.170 | gene GmUGT4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis |
