EC Number |
---|
2.3.1.12 | - |
2.3.1.12 | a recombinant fragment hL2S (containing the second lipoyl domain (L2), second hinge region, E1-binding domain (S) and third hinge region of hE2, residues 128330) is overexpressed in Escherichia coli |
2.3.1.12 | catalytic domain, residues 173-427 |
2.3.1.12 | expressed in Escherichia coli |
2.3.1.12 | expressed in Escherichia coli AG1 cells |
2.3.1.12 | expressed in Escherichia coli BL21(DE3) cells |
2.3.1.12 | expression in Escherichia coli |
2.3.1.12 | expression of subgene for lipoyl domain in Escherichia coli |
2.3.1.12 | expression of the enzymes' L2 domain as GST-fusion protein |
2.3.1.12 | functional overexpression of the enzyme E2 with a single hybrid lipoyl domain per subunit, i.e. 1-lip E2, in strain JRG1342 and BL21(DE3), overexpression of the isolated hybrid lipoyl domain in strain JM 101 |