EC Number |
---|
2.1.1.67 | - |
2.1.1.67 | a 8.7 kb fragment encompassing part of exon III through a portion of intron 6 is subcloned into the pZERO-2 vector |
2.1.1.67 | baculovirus expressed |
2.1.1.67 | cDNA cloned and heterologous expressed in rabbit reticulocytes and wheat germ lysate |
2.1.1.67 | cDNA cloning, heterologous expression in yeast |
2.1.1.67 | enhanced green fluorescent protein-tagged enzyme is expressed in Escherichia coli, Jurkat, Hep-G2, and HEK-293 cells |
2.1.1.67 | expressed in COS-1 cells |
2.1.1.67 | expressed in Escherichia coli BL21-Codon Plus (DE3)-RIL cells |
2.1.1.67 | expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells |
2.1.1.67 | for cloning of the TPMT fragment the TOPO TA cloning kit is used |