EC Number   |
|---|
   2.1.1.229 | co-expression of His-tagged Trm9p with untagged Trm112p in Escherichia coli strain BL21(DE3)pLysS, Trm9p/Trm112p complex formation |
| 2.1.1.229 | expression in Escherichia coli |
| 2.1.1.229 | expression of GST-Trm9 fusion protein in Escherichia coli DH5alpha cells |
| 2.1.1.229 | gene cmoA, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain Rosetta pLysS (DE3) |
| 2.1.1.229 | gene JW5380, expression of wild-type and selenomethinone-labeled MnmC in Escherichia coli strains Rosetta2(DE3) and B834(DE3), respectively |
| 2.1.1.229 | gene mnmC, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 2.1.1.229 | gene mnmC, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), expression of selenomethionine-labeled enzyme in Escherichia coli strain B834 |
| 2.1.1.229 | the FLAG-tagged-ABH8 coding region is cloned into the pBABEpuro or pBABEhygro retroviral vector. HeLa S3 cells are infected with retrovirus |
