EC Number   |
|---|
   1.14.12.18 | - |
| 1.14.12.18 | A central part (amino acid position 268-397 of 458 residues) of the biphenyl dioxygenase large alpha subunit, BphA1, from Pseudomonas pseudoalcaligenes (strain KF707) is exchanged with the corresponding part of BphA1 from Pseudomonas putida strain KF715, to construct hybrid BphA1, BphA1 (amino acid 715-707). When expressed in Escherichia coli together with the bphA2A3A4BC genes from strain KF707, this enzyme is shown to possess activity for degrading both 1-phenylnaphthalene and 2-phenylnaphthalene. |
| 1.14.12.18 | based on analyses with Nicotiana benthamiana plants transiently expressing the biphenyl dioxygenase genes (the two subunit oxygenase (BphAE) containing a Rieske-type ironsulfur cluster and a mononuclear iron center, the Rieske-type ferredoxin (BphF), and the FAD-containing ferredoxin reductase (BphG)) from Burkholderia xenovorans LB400 and transgenic Nicotiana tabacum plants transformed with each of these four genes, it can be shown that each of the three biphenyl dioxygenase components can be produced individually as active protein in tobacco plants |
| 1.14.12.18 | expressed in Escherichia coli |
| 1.14.12.18 | expressed in Escherichia coli C41 (DE3) cells |
| 1.14.12.18 | expressed in Escherichia coli DH11S cells |
| 1.14.12.18 | expressed in Escherichia coli DH5alpha cells |
| 1.14.12.18 | expressed in Escherichia coli JM109 (pJHF108) |
| 1.14.12.18 | expressed in Escherichia coli JM109 cells |
| 1.14.12.18 | expressed in Escherichia coli strain BL21Star |
