EC Number   |
|---|
    2.5.1.9 | optimization of expression in Escherichia coli by construction of a synthetic gene |
| 2.5.1.9 | optimization of expression in Escherichia coli by construction of a synthetic gene, phylogenetic analysis |
| 2.5.1.9 | phylogenetic analysis, highly effective expression in Escherichia coli by construction of a synthetic gene optimized for heterologous expression by exchange of 59 codons |
| 2.5.1.9 | recombinant N-terminal domain of riboflavin synthase is prepared |
| 2.5.1.9 | ribC hyperexpression strain, DNA sequence determination of mutants |
| 2.5.1.9 | wild-type enzyme and the four deletion constructs are individually co-expressed with His6-tagged lumazine synthase in Escherichia coli. Lumazine synthase forms cage complexes with the cognate riboflavin synthase from Aquifex aeolicus when both proteins are co-produced in the cytosol of Escherichia coli. A 12-amino acid-long peptide at the C terminus of riboflavin synthase serves as a specific localization sequence responsible for targeting the guest to the protein compartment |
