EC Number |
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2.4.2.31 | expression of Art 1 in 293T cells as a recombinant fusion protein with the Fc portion of human IgG1 |
2.4.2.31 | expression of GST-tagged wild-type and mutant enzymes in Escherichia coli |
2.4.2.31 | expression of His-tagged ART2 as maltose-binding fusion protein in Escherichia coli strain BL21(DE3), expression of the membrane-bound ART2 in COS1 cells |
2.4.2.31 | expression of isozymes ART1, ART3, ART4, ART5, ART2.2 in Escherichia coli, isozyme ART1synthesized in Escherichia coli, glycosylphosphatidylinositol-anchored ART1 released with phosphatidylinositol-specific phospholipase C from transfected NMU cells, or ART1 expressed endogenously on C2C12 myotubes modifiy arginine 14 on HNP-1 with a secondary site on arginine 24 |
2.4.2.31 | expression of isozymes ART1, ART5, ART2.2 in Escherichia coli, ART1 expressed endogenously on C2C12 myotubes modifies arginine 14 on HNP-1 with a secondary site on arginine 24 |
2.4.2.31 | gene ART3, DNA and amino acid sequence determination and analysis, structural analysis of the untranslated regions, splicing variants differing in the 5'-UTR, genomic organization, expression analysis, recombinant expression in HEK-293T cells |
2.4.2.31 | in COS 7 cells transiently transfected with AT1 cDNA activity is detected in the culture medium. In COS 7 cells transfected with AT2 CDNA activity is found in both culture medium and cell lysate |
2.4.2.31 | mutated amplification products transformed into the Escherichia coli DH 5alpha strain. HEK 293-T cells stably transfected with ART1 or ART3 |
2.4.2.31 | N-terminal Flag-tagged ART4 (amino acids 20-270) cloned (Asp718I/XhoI) into the pSecTagB plasmid. HEK-293T cells stably transfected with the plasmid. C-33A cells stably transfected with an expression plasmid containing the sequence of ART4 encoding amino acids 20-289 |
2.4.2.31 | plasmid pcDNA3-Art7.1 transfected into chicken B cell lymphoma DT40 cells |