EC Number |
---|
1.3.1.3 | gene SRD5B1, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes |
1.3.1.3 | overproduced by the pQE expression system and transformed into Escherichia coli strain B834(DE3) |
1.3.1.3 | pseudogene found |
1.3.1.3 | recombinant expression of AKR1D1 enzyme mutants in HEK-293 cells |
1.3.1.3 | recombinant expression of AKR1D1 enzyme mutants in HEK-293 cells only at low levels possible and with low activities |
1.3.1.3 | Sph1/Sal1 5beta-POR fragment cloned into the pQE vector and transformed into Escherichia coli strain M15(pREP4) |
1.3.1.3 | SPHI /SALI IC5beta-POR fragment cloned into the pQE vector system and transformed into Escherichai coli strain M15(pREP4) |