EC Number   |
|---|
   1.2.4.4 | expression of E1 component in Escherichia coli as a homotetramer, in Escherichia coli only one translational start site for E1beta is recognized and only the 39000 Da product is produced, in addition the N-terminal amino acid of the 39000 Da E1beta subunit is Met instead of Leu |
| 1.2.4.4 | expression of N-and C-terminally His6-tagged component E1b and mutants, and of C-terminally His-tagged lipoic acid-bearing domain, amino acid residues 1-84 of component E2b, in vitro lipoylation of the latter |
| 1.2.4.4 | expression of the lipoic acid-bearing domain of complex component E2 in Escherichia coli strain BL21(DE3) as His-tagged protein |
| 1.2.4.4 | overexpression of bacterial branched-chain alpha-keto acid dehydrogenase multienzyme complex BCKDC, encoded by 4 genes, in Arabidopsis thaliana targeted to chloroplasts, about 7fold increased enzyme activity in the transgenic plants |
| 1.2.4.4 | recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3), coexpression with superoxide dismutase of Thermus thermophilus in Escherichia coli, interaction analysis of 2-oxoisovalerate dehydrogenase alpha subunit with superoxid dismutase of Thermus thermophilus by yeast two hybrid system, recombinant expression in Saccharomyces cerevisiae |
| 1.2.4.4 | region containing the genes for the entire enzyme complex |
