EC Number |
Subunits |
Reference |
---|
4.1.99.1 | ? |
x * 49000, His6-tagged enzyme VcTrpase, SDS-PAGE |
-, 746778 |
4.1.99.1 | ? |
x * 50000, SDS-PAGE |
-, 37366 |
4.1.99.1 | ? |
x * 52000, wild-type enzyme, SDS-PAGE |
-, 727478 |
4.1.99.1 | ? |
x * 52500, SDS-PAGE |
715733 |
4.1.99.1 | ? |
x * 54000, recombinant enzyme, SDS-PAGE |
746632 |
4.1.99.1 | dimer |
inactive dimer formed upon cold incubation, process reversible |
678747 |
4.1.99.1 | dimer |
inactive, formed upon cold incubation or purification |
-, 671054 |
4.1.99.1 | homodimer |
2 * 51000, SDS-PAGE, gel filtration |
-, 715059 |
4.1.99.1 | homotetramer |
4 * 52800, SDS-PAGE |
746651 |
4.1.99.1 | More |
of the two subunits in the asymmetric unit, one is found in the holoform, while the other appears to be in the apoform in a wide-open conformation with two sulfate ions bound in the vicinity of the active site. Enzyme Trpase is flexible in the apoform |
746654 |