EC Number |
Subunits |
Reference |
---|
2.7.4.8 | monomer |
1 * 23000, SDS-PAGE |
642561 |
2.7.4.8 | monomer |
1 * 20548, calculated from amino acid sequence |
642691 |
2.7.4.8 | hexamer |
crystallography |
662657 |
2.7.4.8 | oligomer |
x * 23462, thermodynamic analysis, the enzyme is in equilibrium between a dimer and higher order oligomers, whose relative amounts depend on protein concentration, ionic strength, and the presence of ATP |
662657 |
2.7.4.8 | dimer |
One of the two SaGMK dimers within the crystal asymmetric unit has two monomers in different conformations: an open form with a bound sulfate ion (mimicking the beta-phosphate of ATP) and a closed form with bound GMP and sulfate ion. Each monomer is bound through its P-loop to a sulfate ion. |
671155 |
2.7.4.8 | hexamer |
X-ray diffraction data for EcGMPK in complex with GCV-MP were collected at 100 K from a single crystal on beamline ID23-1 (lambda = 0.980 A) at the European Synchrotron Radiation Facility (Grenoble, France), and is indexed and scaled using XDS and XSCALE to a resolution of 3.16 A. Crystals of EcGMPK GCV-MP belong to space group P41212 and are isomorphous to those of apo-EcGMPK, with 6 molecules related by D3 symmetry in the asymmetric unit. Each monomer was first divided in 3 domains corresponding to the LID, CORE and GMP-binding domains (except for subunit F whose GMP-binding domain is not visible in apo-EcGMPK), which were submitted to rigid body refinement. |
672440 |
2.7.4.8 | More |
GMPKs share a highly conserved structure comprising a GMP-binding domain, a central CORE domain that carries the ATP beta-phosphate binding loop (P-loop) and a LID domain which binds the adenine base of ATP and provides catalytic residues to the phosphoryl transfer reaction. |
672440 |
2.7.4.8 | More |
synaptic scaffolding molecule, S-SCAM, is a synaptic protein, which harbors five or six PSD-95/Discs large/ZO-1, a guanylate kinase, and two WW domains |
675510 |
2.7.4.8 | More |
structural model of microtubule-associated protein-1a. Domain organization of neuronal MAGUKs, overview |
676138 |
2.7.4.8 | dimer |
the recombinant refolded CaVbeta2a-GK is essentially a dimer |
700950 |