EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
6.5.1.1 | 5'-O-(3-Thio)adenosine triphosphate + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
- |
Drosophila melanogaster |
5'-O-(3-Thio)adenosine monophosphate + diphosphate + (deoxyribonucleotide)n+m |
- |
? |
6.5.1.1 | adenosine 5'-[alpha-thio]-triphosphate + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
the reaction rate is slower relative to reactions that use ATP. With adenosine 5'-[alpha-thio]-triphosphateas the cofactor, the ligase displays a significantly higher selectivity for ternary substrates having matched base pairs at the nick site |
Tequatrovirus T4 |
? |
- |
? |
6.5.1.1 | adenosine 5'-[alpha-thio]-triphosphate + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
the reaction rate is slower relative to reactions that use ATP. With ATP-alphaS as the cofactor, the ligase displays a significantly higher selectivity for ternary substrates having matched base pairs at the nick site |
Thermus aquaticus |
? |
- |
? |
6.5.1.1 | adenosine 5'-[beta,gamma-imido]-triphosphate + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
the ATP analogue has a different reactivity in the first reaction to adenylate the probe strand but the overall selectivity does not deviate from those observed when ATP is used |
Thermus aquaticus |
? |
- |
? |
6.5.1.1 | adenosine 5'-[beta,gamma-imido]-triphosphate + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
the ATP analogue has a different reactivity in the first reaction to adenylate the probe strand but the overall selectivity does not deviate from those observed when ATP is used |
Tequatrovirus T4 |
? |
- |
? |
6.5.1.1 | adenosine 5'-[gamma-thio]-triphosphate + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
the ATP analogue has a different reactivity in the first reaction to adenylate the probe strand but the overall selectivity does not deviate from those observed when ATP is used |
Thermus aquaticus |
? |
- |
? |
6.5.1.1 | adenosine 5'-[gamma-thio]-triphosphate + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
the ATP analogue has a different reactivity in the first reaction to adenylate the probe strand but the overall selectivity does not deviate from those observed when ATP is used |
Tequatrovirus T4 |
? |
- |
? |
6.5.1.1 | ADP + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
activity with ADP is slightly lower than with ATP |
Aeropyrum pernix |
AMP + phosphate + (deoxyribonucleotide)n+m |
- |
? |
6.5.1.1 | ADP + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
activity with ADP is slightly lower than with ATP |
Aeropyrum pernix DSM 11879 |
AMP + phosphate + (deoxyribonucleotide)n+m |
- |
? |
6.5.1.1 | AMP + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
the reaction rate is slower relative to reactions that use ATP |
Thermus aquaticus |
? |
- |
? |