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EC Number	Substrates	Commentary Substrates	Organism	Products	Commentary (Products)	Reversibility
5.4.99.42	tRNA uridine31	the enzyme is responsible for modification of cytoplasmic and mitochondrial tRNAs at position 31	Saccharomyces cerevisiae	tRNA pseudouridine31	-	?
5.4.99.42	tRNA uridine31	cytoplasmic and mitochondrial tRNA(Met), mitochondrial tRNA(Lys) and tRNA(Ser). Recombinant His6-tagged Ygr169 protein produced in Escherichia coli is capable of forming pseudouridine31 in vitro using tRNAs extracted from the DELTAYGR169c yeast cells as substrates. The enzyme does not catalyse the pseudouridylation of uridine31	Saccharomyces cerevisiae	tRNA pseudouridine31	-	?

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Release 2023.1 (January 2023)