EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
    4.2.1.157 | (R)-2-hydroxy-4-methylpentanoyl-CoA |
- |
Mycobacterium tuberculosis |
4-methylpent-2-enoyl-CoA + H2O |
- |
? |
| 4.2.1.157 | (R)-2-hydroxy-4-methylpentanoyl-CoA |
i.e. (R)-2-hydroxyisocaproyl-CoA. The enzyme is involved in the reductive branch of L-leucine fermentation |
Clostridioides difficile |
4-methylpent-2-enoyl-CoA + H2O |
i.e. isocaprenoyl-CoA |
? |
| 4.2.1.157 | (R)-2-hydroxy-4-methylpentanoyl-CoA |
the enzyme is involved in fermentation of L-leucine |
Clostridioides difficile |
4-methylpent-2-enoyl-CoA + H2O |
- |
? |
| 4.2.1.157 | (R)-2-hydroxy-4-methylpentanoyl-CoA |
i.e. (R)-2-hydroxyisocaproyl-CoA. The enzyme is specific for the R-isomer. The reduced activator transfers one electron to the dehydratase concomitant with hydrolysis of ATP. The activated dehydratase is separated from the activator and ATP. It catalyzes about 10000 dehydration turnovers until the enzyme becomes inactive. Adding activator, ATP, MgCl2, dithionite and dithioerythritol reactivates the enzyme. The enzymatic elimination of H2O probably occurs in syn-fashion. In assays using (E)-isocaprenoyl-CoA as substrate, no activity can be observed suggesting that the dehydration is irreversible under these conditions or the Z-isomer is the correct product |
Clostridioides difficile |
4-methylpent-2-enoyl-CoA + H2O |
i.e. isocaprenoyl-CoA |
? |
| 4.2.1.157 | (R)-2-hydroxy-4-methylpentanoyl-CoA |
the catalyzed reaction, an atypical alpha/beta-dehydration, depends on the reductive formation of ketyl radicals on the substrate generated by injection of a single electron from the ATP-dependent activator protein |
Clostridioides difficile |
4-methylpent-2-enoyl-CoA + H2O |
- |
? |
| 4.2.1.157 | (R)-2-hydroxy-4-methylpentanoyl-CoA |
- |
Mycobacterium tuberculosis ATCC 25618/ H37Rv |
4-methylpent-2-enoyl-CoA + H2O |
- |
? |
| 4.2.1.157 | (R)-2-hydroxy-4-methylpentanoyl-CoA |
- |
Mycobacterium tuberculosis ATCC 25177 / H37Ra |
4-methylpent-2-enoyl-CoA + H2O |
- |
? |
| 4.2.1.157 | more |
1H-NMR analysis of the unsaturations in the mycolic acid of different Mycobacterium tuberculosis wild-type and mutant strains, overview |
Mycobacterium tuberculosis |
? |
- |
? |
| 4.2.1.157 | more |
1H-NMR analysis of the unsaturations in the mycolic acid of different Mycobacterium tuberculosis wild-type and mutant strains, overview |
Mycobacterium tuberculosis ATCC 25618/ H37Rv |
? |
- |
? |
| 4.2.1.157 | more |
1H-NMR analysis of the unsaturations in the mycolic acid of different Mycobacterium tuberculosis wild-type and mutant strains, overview |
Mycobacterium tuberculosis ATCC 25177 / H37Ra |
? |
- |
? |
