EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   3.2.2.29 | 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O |
- |
Drosophila melanogaster |
3,N4-ethenocytosine + double-stranded DNA with abasic site |
- |
? |
| 3.2.2.29 | 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O |
- |
Homo sapiens |
3,N4-ethenocytosine + double-stranded DNA with abasic site |
- |
? |
| 3.2.2.29 | 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O |
3,N4-ethenocytosine is recognized and efficiently excised by hTDG. The enzyme may be responsible for the repair of this mutagenic lesion in vivo and be important contributors to genetic stability |
Homo sapiens |
3,N4-ethenocytosine + double-stranded DNA with abasic site |
- |
? |
| 3.2.2.29 | 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O |
3,N4-ethenocytosine is recognized and efficiently excised from the 3,N4-ethenocytosine/G duplex oligonucleotide, when this residue is situated opposite to G. 26.5% of the activity measured with 3,N4-ethenocytosine mismatches is observed with 3,N4-ethenocytosine/A mismatches, 71% with 3,N4-ethenocytosine/A mismatches |
Homo sapiens |
3,N4-ethenocytosine + double-stranded DNA with abasic site |
- |
? |
| 3.2.2.29 | 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O |
ethenocytosine base-paired with guanine within a CpG site (i.e. CpG-ethenocytosine-DNA) is by far the best substrate. The next best substrates are DNA duplexes containing TpG/ethenocytosine, GpG/ethenocytosine, and CpG/T. The worst substrates are DNA duplexes containing ApG/ethenocytosine and TpG/T. DNA containing ethenocytosine is bound much more tightly than DNA containing a G/T mismatch |
Homo sapiens |
3,N4-ethenocytosine + double-stranded DNA with abasic site |
- |
? |
| 3.2.2.29 | 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O |
removes 3,N4-ethenocytosine from G/3,N4-ethenocytosine and A/3,N4-ethenocytosine mismatches |
Homo sapiens |
3,N4-ethenocytosine + double-stranded DNA with abasic site |
- |
? |
| 3.2.2.29 | 5-bromocytosine-mismatched double-stranded DNA + H2O |
hTDG readily excises cytosine analogues with improved leaving ability, including 5-fluorocytosine, 5-bromocytosine, and 5-hydroxycytosine, indicating that cytosine has access to the active site. hTDG specificity depends on N-glycosidic bond stability, and the discrimination against cytosine is due largely to its very poor leaving ability rather than its exclusion from the active site |
Homo sapiens |
5-bromouracil + double-stranded DNA with abasic site |
- |
? |
| 3.2.2.29 | 5-bromouracil-mismatched double-stranded DNA + H2O |
potential role played by human TDG in the cytotoxic effects of 5-chlorouracil and 5-bromouracil incorporation into DNA, which can occur under inflammatory conditions |
Homo sapiens |
5-bromouracil + double-stranded DNA with abasic site |
- |
? |
| 3.2.2.29 | 5-bromouracil-mismatched double-stranded DNA + H2O |
in addition to uracil and thymine, the protein can also remove 5-bromouracil from mispairs with guanine |
Homo sapiens |
5-bromouracil + double-stranded DNA with abasic site |
- |
? |
| 3.2.2.29 | 5-bromouracil-mismatched double-stranded DNA + H2O |
paired with guanine |
Homo sapiens |
5-bromouracil + double-stranded DNA with abasic site |
- |
? |
