EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.5.1.2 | 1'-methylthiamine + aniline |
22% of activity with thiamine |
Cyprinus carpio |
4-methyl-5-(2-hydroxyethyl)-thiazole + 4-amino-5-(anilinomethyl)-1,2-dimethylpyrimidin-1-ium |
- |
? |
2.5.1.2 | 2'-ethylthiamine + aniline |
63% of activity with thiamine |
Cyprinus carpio |
4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(anilinomethyl)-2-ethylpyrimidin-4-amine |
- |
? |
2.5.1.2 | 2-(1-hydroxyethyl)thiamine + aniline |
92% of activity with thiamine |
Cyprinus carpio |
4-methyl-2-(1-hydroxyethyl)-5-(2-hydroxyethyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine |
- |
? |
2.5.1.2 | 5-chloroethylthiamine + aniline |
67% of activity with thiamine |
Cyprinus carpio |
4-methyl-5-chloroethyl-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine |
- |
? |
2.5.1.2 | 5-nor-thiamine + aniline |
88% of activity with thiamine |
Cyprinus carpio |
4-methyl-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine |
- |
? |
2.5.1.2 | more |
no activity with 4'-deaminothiamine and 4'-oxythiamine suggest that 4'-amino group is essential for enzyme activity |
Cyprinus carpio |
? |
- |
? |
2.5.1.2 | more |
primary substrates inactivate the enzyme at 0.006 mM and secondary substrates or acceptor bases reactivate the enzyme at 10 mM, each substrate at high concentrations eliminates the inactivation of the other substrate |
Paenibacillus thiaminolyticus |
? |
- |
? |
2.5.1.2 | more |
primary substrates inactivate the enzyme at 0.006 mM and secondary substrates or acceptor bases reactivate the enzyme at 10 mM, each substrate at high concentrations eliminates the inactivation of the other substrate |
Paenibacillus thiaminolyticus YUSM I00I |
? |
- |
? |
2.5.1.2 | O-(beta-D-galactosyl)thiamine + aniline |
63% of activity with thiamine |
Cyprinus carpio |
4-methyl-5-(2-hydroxyethyl-O-beta-D-galactosyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine |
- |
? |
2.5.1.2 | O-benzoylthiamine + aniline |
91% of activity with thiamine |
Cyprinus carpio |
2-(4-methyl-1,3-thiazol-5-yl)ethyl benzoate + 5-(anilinomethyl)-2-methylpyrimidin-4-amine |
- |
? |