EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.4.1.B77 | more |
all the dextrans tested are branched by BRS-A and show 37% alpha-(1->2) linkages |
Leuconostoc citreum |
? |
- |
? |
2.4.1.B77 | more |
enzyme additionally has transglucosylation activity towards flavonoids including quercetin, luteolin, apigenin, morin, chrysin and naringenin |
Leuconostoc citreum |
? |
- |
? |
2.4.1.B77 | more |
alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview. The pattern of the molar mass increase depends on the branching degree and the branching type, probably because of the different conformations due to the different flexibility and hindrance introduced by either the alpha(1->2) or the alpha(1->3) linkages |
Leuconostoc citreum |
? |
- |
- |
2.4.1.B77 | more |
alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview. The pattern of the molar mass increase depends on the branching degree and the branching type, probably because of the different conformations due to the different flexibility and hindrance introduced by either the alpha(1->2) or the alpha(1->3) linkages |
Leuconostoc citreum NRRL B-742 |
? |
- |
- |
2.4.1.B77 | more |
all the dextrans tested are branched by BRS-A and show 37% alpha-(1->2) linkages |
Leuconostoc citreum NRRL B-1299 |
? |
- |
? |
2.4.1.B77 | more |
enzyme additionally has transglucosylation activity towards flavonoids including quercetin, luteolin, apigenin, morin, chrysin and naringenin |
Leuconostoc citreum NRRL B-1299 |
? |
- |
? |
2.4.1.B77 | more |
alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview. The pattern of the molar mass increase depends on the branching degree and the branching type, probably because of the different conformations due to the different flexibility and hindrance introduced by either the alpha(1->2) or the alpha(1->3) linkages |
Leuconostoc citreum NRRL B-1299 |
? |
- |
- |
2.4.1.B77 | sucrose + (1->6)-alpha-D-glucan |
- |
Leuconostoc citreum |
D-fructose + (1->6)-alpha-D-glucan containing a (1->2)-alpha-D-glucose branch |
- |
? |
2.4.1.B77 | sucrose + (1->6)-alpha-D-glucan |
- |
Leuconostoc citreum NRRL B-742 |
D-fructose + (1->6)-alpha-D-glucan containing a (1->2)-alpha-D-glucose branch |
- |
? |
2.4.1.B77 | sucrose + (1->6)-alpha-D-glucan |
- |
Leuconostoc citreum NRRL B-1299 |
D-fructose + (1->6)-alpha-D-glucan containing a (1->2)-alpha-D-glucose branch |
- |
? |