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EC Number Substrates Commentary Substrates Organism Products Commentary (Products) Reversibility
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.764 S-adenosyl-L-methionine + quercetin whole-cell biocatalysis using CdFOMT5 expressed in Escherichia coli cells is performed using quercetin as a substrate, and 3,3',5,7-tetramethylated quercetin is obtained as the final product Citrus depressa 4 S-adenosyl-L-homocysteine + 3,3',5,7-tetramethoxyquercetin - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.76more the enzyme does not methylate 3',4',5'-O-trimethylmyricetin, 3,7,3',4'-O-methylquercetin, 3-methylquercetin, and 3,7,4'-O-trimethylkaempferol. The enzyme transfers a methyl group only to the 3-position hydroxyl Solanum habrochaites ? - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.76more the flavonoid-O-methyltransferase from Citrus depressa has a broad substrate specificity and regioselectivity. Isozyme CdFOMT5 exhibits O-methyltransferase activity for quercetin, naringenin, (-)-epicatechin, and equol using S-adenosyl-L-methionine (SAM) as a methyl donor. The recombinant CdFOMT5 CdFOMT5 can catalyze the O-methylation of at least three hydroxyl groups of quercetin, and di- or tri-O-methylated quercetin products are obtained by this enzymatic reaction. CdFMOT5 prefers flavonol (3-hydroxyflavone) to other flavonoid structures. Thus, substrate structure, especially the C-ring in flavonoids, may strongly affect the substrate preference, including regioselectivity, of CdFOMT5 Citrus depressa ? - -
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.76S-adenosyl-L-methionine + 3',5'-O-dimethylmyricetin 18% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + 3,3',5'-O-trimethylmyricetin - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.76S-adenosyl-L-methionine + 3'-O-methylmyricetin 64% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + 3,3'-O-dimethylmyricetin - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.76S-adenosyl-L-methionine + 3'-O-methylmyricetin i.e. laricitrin, 43% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + 3,3'-O-dimethylmyricetin - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.76S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone - Arabidopsis thaliana S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.76S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone - Chrysosplenium americanum S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.76S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone - Citrofortunella microcarpa S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.76S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone - Oryza sativa S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone - ?
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