EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   2.1.1.221 | more |
substrate specificity analysis in vivo, overview |
Homo sapiens |
? |
- |
? |
| 2.1.1.221 | more |
there are 19 unique tRNA species that contain a G at position 9 in yeast, and whose fully modified sequence is known, only 9 of these tRNA species are modified with m1G9 in wild-type cells. The in vitro methylation activity of yeast Trm10 is not sufficient to explain the observed pattern of modification in vivo, as additional tRNA species are substrates for Trm10 m1G9 methyltransferase activity, substrate specificity analysis in vivo, overview |
Saccharomyces cerevisiae |
? |
- |
? |
| 2.1.1.221 | more |
no activity of the enzyme with three sptRNAGly mutants at Position 9 (G9A, G9C, and G9U) |
Saccharomyces cerevisiae |
? |
- |
? |
| 2.1.1.221 | more |
no activity of the enzyme with three sptRNAGly mutants at Position 9 (G9A, G9C, and G9U) |
Schizosaccharomyces pombe |
? |
- |
? |
| 2.1.1.221 | more |
substrate specificity analysis and comparison of hTRMT10A and hTRMT10B enzymes, overview. hTRMT10A and hTRMT10B catalyze methylation of different tRNA substrates. hTRMT10A displays robust activity on every tested G9-containing tRNA from either humans or Saccharomyces cerevisiae, similar to Saccharomyces cerevisiae ScTrm10. hTRMT10B, and not hTRMT10A, is indeed capable of catalyzing m1A9 methylation on tRNAAsp in vitro, while hTRMT10B has the sole responsibility for generating the m1A9 modification. hTRMT10A displays higher in vitro catalytic rates than hTRMT10B |
Homo sapiens |
? |
- |
- |
| 2.1.1.221 | more |
the human Trm10C enzyme is bifunctional and methylates adenine9 (EC 2.1.1.218) and guanine9 (EC 2.1.1.221) residues in tRNA |
Homo sapiens |
? |
- |
- |
| 2.1.1.221 | more |
the human Trmt10A enzyme does not exhibit activity on adenine9 residues in tRNA, no activity of EC 2.1.1.218 |
Homo sapiens |
? |
- |
- |
| 2.1.1.221 | more |
the Trm10 enzyme from Thermococcus kodakarensis is bifunctional and methylates adenine9 (EC 2.1.1.218) and guanine9 (EC 2.1.1.221) residues in tRNA |
Thermococcus kodakarensis |
? |
- |
- |
| 2.1.1.221 | more |
the yeast Trm10 enzyme does not exhibit activity on adenine9 residues in tRNA, no activity of EC 2.1.1.218 |
Saccharomyces cerevisiae |
? |
- |
- |
| 2.1.1.221 | more |
the yeast Trm10 enzyme does not exhibit activity on adenine9 residues in tRNA, no activity of EC 2.1.1.218 |
Schizosaccharomyces pombe |
? |
- |
- |
