EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.4.7.1 | 2 L-glutamate + 2 oxidized ferredoxin |
- |
Arabidopsis thaliana |
L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+ |
- |
? |
1.4.7.1 | 2 L-glutamate + 2 oxidized ferredoxin |
- |
Synechocystis sp. |
L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+ |
- |
? |
1.4.7.1 | 2 L-glutamate + 2 oxidized ferredoxin |
overall reaction. The enzyme is involved in the ammonium assimilation pathway which enables Haloferax mediterranei to thrive in media with low ammonium concentration or containing just nitrate as single nitrogen source |
Haloferax mediterranei |
L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+ |
- |
? |
1.4.7.1 | 2 L-glutamate + 2 oxidized ferredoxin |
overall reaction. The enzyme is involved in the ammonium assimilation pathway which enables Haloferax mediterranei to thrive in media with low ammonium concentration or containing just nitrate as single nitrogen source |
Haloferax mediterranei ATCC 33500 |
L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+ |
- |
? |
1.4.7.1 | L-glutamate + oxidized iodonitrotetrazolium |
enzyme exhibits L-glutamate:iodonitrotetrazolium oxidoreductase activity at pH 7.5-9.5 |
Synechocystis sp. |
2-oxoglutarate + NH3 + reduced iodonitrotetrazolium |
- |
? |
1.4.7.1 | L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+ |
- |
Synechocystis sp. |
2 L-glutamate + 2 oxidized ferredoxin |
- |
? |
1.4.7.1 | L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+ |
no activity is detected in presence of either 5 mM NADH or 0.5 mM NADPH. Methyl viologen at 20 mM and 0.004 mM ferredoxin I are also tested under the same conditions: activity with 0.004 mM ferredoxin I as reducing agent is approximately 3fold higher than that measured with 20 mM methyl viologen. For the other ferrodoxin isoform found in Haloferax mediterranei, ferrodoxin II, the activity is approximately 8fold lower than that for ferredoxin I |
Haloferax mediterranei |
2 L-glutamate + 2 oxidized ferredoxin |
- |
? |
1.4.7.1 | L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+ |
no activity is detected in presence of either 5 mM NADH or 0.5 mM NADPH. Methyl viologen at 20 mM and 0.004 mM ferredoxin I are also tested under the same conditions: activity with 0.004 mM ferredoxin I as reducing agent is approximately 3fold higher than that measured with 20 mM methyl viologen. For the other ferrodoxin isoform found in Haloferax mediterranei, ferrodoxin II, the activity is approximately 8fold lower than that for ferredoxin I |
Haloferax mediterranei ATCC 33500 |
2 L-glutamate + 2 oxidized ferredoxin |
- |
? |
1.4.7.1 | L-glutamine + 2-oxoglutarate + 2 reduced methyl viologen |
reductant: Na2S2O4 |
Arabidopsis thaliana |
2 L-glutamate + 2 oxidized methyl viologen |
- |
? |
1.4.7.1 | L-glutamine + 2-oxoglutarate + 2 reduced methyl viologen + 2 H+ |
- |
Synechocystis sp. PCC 6803 |
2 L-glutamate + 2 oxidized methyl viologen |
- |
? |