EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   3.2.1.129 | longchain alpha 2,8-linked polysialic acid + H2O |
minimum substrate is tetrameric polysialic acid, processive enzyme activity on oligomers larger than that, confirmation by H NMR spectroscopy and anion-exchange chromatography |
Escherichia phage K1F |
oligomers of alpha 2,8-linked polysialic acid |
major product is an oligomer consisting of 3 monomers in wild-type and cleavage mutant S911A, in binding site mutant R837A/S848A more random oligomers are produced |
? |
| 3.2.1.129 | more |
the endo-sialidase requires the occupation of a minimum of three subsites by sialic acid for efficient catalysis, so neither monomer (trifluoromethylumbelliferyl sialoside) nor dimer (trifluoromethylumbelliferyl sialobioside) are hydrolyzed by endoNF |
Escherichia phage K1F |
? |
- |
? |
| 3.2.1.129 | more |
the endosialidase is a bifunctional enzyme with high activity against alpha-2,8- and low activity against alpha-2,9-linkages in a polySia chain |
Enterobacteria phage phi92 |
? |
- |
? |
| 3.2.1.129 | oligo(sialic) acid + H2O |
- |
Escherichia phage K1F |
fragments of oligo(sialic) acid |
- |
? |
| 3.2.1.129 | poly(sialic) acid + H2O |
- |
Escherichia phage K1F |
? |
- |
? |
| 3.2.1.129 | poly(sialic) acid + H2O |
- |
Vectrevirus K1E |
? |
- |
? |
| 3.2.1.129 | poly(sialic) acid + H2O |
- |
Enterobacteria phage K1A |
? |
- |
? |
| 3.2.1.129 | poly(sialic) acid + H2O |
all sialic acid residues (Sia1-5) are bound by endoNF |
Bacteriophage sp. |
? |
- |
? |
| 3.2.1.129 | poly(sialic) acid + H2O |
- |
Escherichia phage K1F |
fragments of poly(sialic) acid |
- |
? |
| 3.2.1.129 | poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O |
- |
Escherichia phage K1F |
fragments of poly(sialic) acid or oligo(sialic) acids |
- |
? |
