EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
    2.3.1.30 | -999 |
- |
- |
486754, 486756, 486761 |
| 2.3.1.30 | -999 |
- |
50 mM Tris-HCl, pH 7.5, csat mutant (deficient in cytosolic enzyme), total serine acetyltransferase activity reduced to 71% compared to wild-type in leaves, to 68% in roots |
706161 |
| 2.3.1.30 | -999 |
- |
50 mM Tris-HCl, pH 7.5, dmsat mutant (deficient in cytosolic and plastidic enzyme), total serine acetyltransferase activity reduced to 80% compared to wild-type in roots, no significant reduction in leaves |
706161 |
| 2.3.1.30 | -999 |
- |
50 mM Tris-HCl, pH 7.5, psat mutant (deficient in plastidic enzyme), without significant total serine acetyltransferase alterations in leaves and roots |
706161 |
| 2.3.1.30 | -999 |
- |
at acetyl-CoA concentration 15fold higher than L-serine concentration, a nonproductive ternary complex of enzyme-CoA-L-serine is formed, kinetics |
486778 |
| 2.3.1.30 | -999 |
- |
coupled assay system |
486755, 486760 |
| 2.3.1.30 | -999 |
- |
coupling reaction with cysteine synthase, 50 mM Tris-HCl, pH 8.0, 0.4 mM acetyl-CoA, 4 mM L-serine, 5 mM Na2S, 10 mM DTT, recombinant Entamoeba histolytica cysteine synthase 3, 37°C (amount of L-cysteine produced) |
705594 |
| 2.3.1.30 | -999 |
- |
recombinant Arabidopsis serine acetlytransferase activity in transgenic Lupinus angustifolius embryos of the T2 and T4 plant generation is increased severalfold over controls (wild-type and transgenic sunflower seed albumin lupins), 5-12fold and about 5fold in 5F1 (in vitro 4fold over wild-type), 24-31fold and about 20fold in 3A3 (in vitro 12fold over wild-type), 49fold and about 40fold in 5B3 (in vitro 30fold over wild-type), control enzyme activity is roughly about 5 nmol/min/mg protein, correlation to the concentration of key intermediates of the sulphur amino acid biosynthesis (O-acetylserine, free cysteine, glutathione) |
704896 |
| 2.3.1.30 | 0.0000304 |
- |
substrate L-serine, pH 7.5, 37°C, recombinant mutant D94A |
757611 |
| 2.3.1.30 | 0.0000812 |
- |
substrate L-serine, pH 7.5, 37°C, recombinant mutant R129A |
757611 |
