EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
5.1.2.2 | 0.056 |
- |
(S)-2-chloromandelate |
wild-type enzyme, pH 7.5, 25°C |
746856 |
5.1.2.2 | 0.061 |
- |
(R)-2-chloromandelate |
wild-type enzyme, pH 7.5, 25°C |
746856 |
5.1.2.2 | 0.106 |
- |
(R)-2-chloromandelate |
mutant Y45F, pH 7.5, 25°C |
746856 |
5.1.2.2 | 0.118 |
- |
(R)-2-chloromandelate |
mutant V22I, pH 7.5, 25°C |
746856 |
5.1.2.2 | 0.121 |
- |
(R)-2-chloromandelate |
mutant V29I, pH 7.5, 25°C |
746856 |
5.1.2.2 | 0.122 |
- |
(S)-2-chloromandelate |
mutant Y45F, pH 7.5, 25°C |
746856 |
5.1.2.2 | 0.124 |
- |
(S)-2-chloromandelate |
mutant V22I, pH 7.5, 25°C |
746856 |
5.1.2.2 | 0.128 |
- |
(S)-2-chloromandelate |
mutant V29I, pH 7.5, 25°C |
746856 |
5.1.2.2 | 0.15 |
- |
(R)-2-chloromandelate |
mutant V22I/Y45F, pH 7.5, 25°C |
746856 |
5.1.2.2 | 0.159 |
- |
(S)-2-chloromandelate |
mutant V22I/V29I, pH 7.5, 25°C |
746856 |