EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
4.1.2.14 | 0.014 |
- |
4-hydroxy-2-oxooctonate |
wild-type, pH 7.5, 25°C |
726975 |
4.1.2.14 | 0.06 |
- |
4-hydroxy-2-oxooctonate |
mutant S184F, pH 7.5, 25°C |
726975 |
4.1.2.14 | 0.113 |
- |
4-hydroxy-2-oxooctonate |
mutant T161S, pH 7.5, 25°C |
726975 |
4.1.2.14 | 0.33 |
- |
(4S)-2-oxo-4-hydroxy-4-(2'-pyridyl)butanoate |
wild-type, pH 7.5, 25°C |
726975 |
4.1.2.14 | 0.72 |
- |
4-hydroxy-2-oxooctonate |
mutant T161S/S18L, pH 7.5, 25°C |
726975 |
4.1.2.14 | 3.3 |
- |
(4S)-2-oxo-4-hydroxy-4-(2'-pyridyl)butanoate |
mutant S184F, pH 7.5, 25°C |
726975 |
4.1.2.14 | 4.1 |
- |
(4S)-2-oxo-4-hydroxy-4-(2'-pyridyl)butanoate |
mutant T161S, pH 7.5, 25°C |
726975 |
4.1.2.14 | 24 |
- |
2-dehydro-3-deoxy-6-phospho-D-gluconate |
mutant S184F, pH 7.5, 25°C |
726975 |
4.1.2.14 | 45 |
- |
2-dehydro-3-deoxy-6-phospho-D-gluconate |
mutant T161S/S184F, pH 7.5, 25°C |
726975 |
4.1.2.14 | 61 |
- |
(4S)-2-oxo-4-hydroxy-4-(2'-pyridyl)butanoate |
mutant T161S/S184F, pH 7.5, 25°C |
726975 |