   3.4.17.8 | physiological function |
deletion of two DD-CPase genes, msmeg_2433 and msmeg_2432, both individually and in combination. The single DD-CPase gene deletions have no significant impact on the mycobacterial physiology. In the double-deletion mutant, the predominance in peptidoglycan cross-link formation is shifted from 3-3 cross-links to 4-3, cell surface glycopeptidolipid expression is reduced, and susceptibility to beta-lactams and antitubercular agents is enhanced. The survival rate of the double mutant within murine macrophages is higher than that of the parent. The complementation with any one of the DD-CPase genes can restore the wild-type phenotype |
-, 754019 |
