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EC Number General Information Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.97malfunction a Haloferax volcanii DELTAtgtA2 derivative demonstrates that tRNA from the mutant strain lacks G+ and instead accumulates preQ0 -, 719859
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.97physiological function archaeosine (G+), 7-formamidino-7-deazaguanosine, is an archaea-specific modified nucleoside found at the 15th position of tRNAs. In Euryarchaeota, 7-cyano-7-deazaguanine (preQ0)-containing tRNA (q0N-tRNA), synthesized by archaeal tRNA-guanine transglycosylase (ArcTGT), is converted to G+-containing tRNA (G+-tRNA) by the paralogue of ArcTGT, ArcS. Several euryarchaeal ArcSs have lysine transfer activity to q0N-tRNA to form q0kN-tRNA, which has a preQ0 lysine adduct as a base. ArcS forms a robust complex with a radical S-adenosylmethionine (SAM) enzyme named RaSEA. The ArcS-RaSEA complex anaerobically converts q0N-tRNA to G+-tRNA in the presence of SAM and lysine via q0kN-tRNA. It is proposed that ArcS and RaSEA should be considered an archaeosine synthase alpha-subunit (lysine transferase) and beta-subunit (q0kN-tRNA lyase), respectively -, 759819
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.97metabolism archaeosine biosynthesis pathway, overview -, 759819
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.97physiological function ArcS catalyzes the final step in the G+ pathway, the conversion of preQ0-tRNA to G+-tRNA, in Euryarchaeota 717018
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.97more G+ can be tolerated in Escherichia coli at position 34 in normally Q-containing tRNA when recombinant GAT-QueC and QueF are introduced 717018
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.97metabolism GAT-QueC also catalyzes biosynthesis of G+-tRNA, pathways, overview 717018
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.97more presence of the 7-deazaguanosine derivative archaeosine, i.e. G+, at position 15 in tRNA is one of the diagnostic molecular characteristics of the archaea -, 719859
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.97malfunction recombinant expression of the arcTGT orthologue from Thermoplasma acidophilum in the hyperthermophilic euryarchaeon Thermococcus kodakarensis arcS-deletion strain and functional complementation. Less TkRaSEA is obtained from the extract containing TaArcS compared to TkArcS -, 759819
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.97evolution specific Archaea such as Sulfolobus tokodaii have retained ArcS in addition to GAT-QueC, overview 717018
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.97evolution structure-based alignments comparing arcTGT and TgtA2 reveal that TgtA2 lacks key arcTGT catalytic residues and contains an additional module. Members of the TgtA2 and arcTGT family do not perfectly co-distribute -, 719859
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