EC Number   |
General Information |
Reference |
|---|
   1.16.3.4 | metabolism |
the three copper sites play related but distinct roles in CueO oxidase activities. The internal Cu5 site is part of the essential electron transfer pathway connecting surface-exposed sites Cu6 and Cu7 to site T1. Both Cu6 and Cu7 are dominant substrate-docking-oxidation sites on the protein surface. The two surface-exposed sites Cu6 and Cu7 are the direct substrate-docking-oxidation sites for both oxidase functions and buried site Cu5 channels electrons from the oxidations to the Type 1 site of the multicopper oxidase machinery |
763429 |
| 1.16.3.4 | physiological function |
presence of CueO in the periplasm protects alkaline phosphatase from copper-induced damage |
762691 |
| 1.16.3.4 | physiological function |
Salmonella typhimurium CueO mutant is more sensitive to copper ions than wild-type. In a murine model of infection, the CueO mutant is significantly attenuated, although there is no significant difference in recovery from Peyer's patches and mesenteric lymph nodes. The intracellular survival of the CueO mutant in unprimed or gamma-interferon-primed murine macrophages is not statistically different from that of wild-type Salmonella typhimurium. The CueO mutant does not show greater sensitivity to hydrogen peroxide and its sensitivity to copper ions is not affected by siderophores |
-, 763131 |
| 1.16.3.4 | physiological function |
the cue pathway, which includes a copper exporter, CopA, and a periplasmic oxidase, CueO, is the primary aerobic system for copper tolerance. During anaerobic growth, however, copper toxicity increases, and the independent cus copper exporter is also necessary for full copper tolerance. Oxidase activity is abolished in the strain lacking CueO regardless of the presence of added copper, deletion of CueO does not affect copper sensitivity relative to that of wild-type strains |
763233 |
| 1.16.3.4 | physiological function |
the enzyme is required for copper homeostasis in Escherichia coli |
-, 763245 |
| 1.16.3.4 | physiological function |
the enzyme is required for iron homeostasis. It also plays a major role in maintaining the cuprous/cupric redox balance |
-, 763026 |
| 1.16.3.4 | physiological function |
when expressed in an Escherichia coli CueO knock-out strain, enzyme exhibits phenol oxidase activity in vivo and enhances the copper tolerance of the strain |
763449 |
