3.1.3.32 | hydrolysis |
although unable to dephosphorylate a 3'-PO4-terminated single strand, the enzyme is equally adept at removing the 3'-PO4 from an internal nick or gap in duplex DNA, a recessed 3' end of a 5'-tailed primer-template, or a blunt DNA duplex with no single-strand tail |
680642 |