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EC Number Reaction Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.7.8.17UDP-N-acetyl-D-glucosamine + lysosomal-enzyme D-mannose = UMP + lysosomal-enzyme N-acetyl-D-glucosaminyl-phospho-D-mannose - -
Display the word mapDisplay the reaction diagram Show all sequences 2.7.8.17UDP-N-acetyl-D-glucosamine + lysosomal-enzyme D-mannose = UMP + lysosomal-enzyme N-acetyl-D-glucosaminyl-phospho-D-mannose the alpa/beta subunits of GlcNAc-1-phosphotransferase recognize and bind the conformation-dependent protein determinant of acid hydrolases as well as critical lysines. The subunits then mediate the catalytic function of the transferase. Interaction of the alpa/beta subunits with the protein determinant of acid hydrolases stimulates the catalytic function of the transferase. The gamma subunit enhances the rate of GlcNAc-P transfer to the oligosaccharides of acid hydrolase acceptors. The requirement for the gamma subunit to achieve optimal levels of phosphorylation varies greatly among the different acid hydrolases, and in some instances the gamma subunit is dispensable 725407
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