EC Number |
---|
5.1.3.5 | - |
5.1.3.5 | Ni-NTA column chromatography |
5.1.3.5 | Ni-Sepharose column chromatography |
5.1.3.5 | purification of the native enzyme from pea sprouts by ion exchange chromatography and gel filtration, further purification on a hydroxyapatite column, purification of recombinant enzymes by ion exchange chromatography |
5.1.3.5 | purification of the recombinant Arabidopsis enzyme by Ni-NTA affinity chromatography |
5.1.3.5 | recombinant His-tagged truncated inactive enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |