EC Number |
---|
5.1.3.3 | - |
5.1.3.3 | enzyme form I and II from kidney and enzyme form II from liver |
5.1.3.3 | multiple forms: I, II, III, and IV. Two major forms: I and II are purified |
5.1.3.3 | native enzyme 8.35fold from liver by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration |
5.1.3.3 | Ni-NTA affinity column chromatography and Superdex 200 gel filtration |
5.1.3.3 | NiNTA affinity chromatography |
5.1.3.3 | NiNTA affinity column chromatography |
5.1.3.3 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
5.1.3.3 | recombinant His6-tagged enzyme from Escherichia coli strain MG1655 |
5.1.3.3 | UDPgalactose 4-epimerase is a bifunctional enzyme with aldose 1-epimerase activity. The active sites for the two enzymatic activities are located in different regions of the epimerase homoenzyme |