EC Number   |
|---|
   3.7.1.7 | - |
| 3.7.1.7 | recombinant enzyme 1-67fold from Pichia pastoris strain GS115 culture supernatant by ultrafiltration, and hydrophobic interaction and cation exchange chromatography |
| 3.7.1.7 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 trxB (DE3) by nickel affinity chromatography and digestion by TEV protease to remove the His-tagged thioredoxin, followed by a second nickel affinity chromatography, and anion exchange chromatography and ultrafiltration |
| 3.7.1.7 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
| 3.7.1.7 | to homogeneity, 5-9fold, gel filtration |
| 3.7.1.7 | using a nickel affinity HiTRAP agarose column and a S200 16/60 Sephadex column |
